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anti il4 neutralizing antibody  (Bio X Cell)


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    Structured Review

    Bio X Cell anti il4 neutralizing antibody
    Anti Il4 Neutralizing Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+il4/InVivoMAb+anti-mouse+IL-4/pm42816626-736-8-12
    Average 96 stars, based on 188 article reviews
    anti il4 neutralizing antibody - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: A20’s Linear Ubiquitin Binding Motif Restrains Pathogenic Activation of TH17/22 cells and IL-22 Driven Enteritis
    Article Snippet: .. Cells were subsequently differentiated into TH17 cells in plates pre-coated with 5 ug/mL anti-CD3 in TH17 medium (Immunocult XF serum-free medium with 50 uM beta-mercaptoethanol, 30 ng/mL recombinant human IL-6 (Proteintech HZ-1019), 2.5 ng/mL recombinant human TGFb1 (PeproTech 100-21), 10 ng/mL recombinant human IL-1β (Proteintech HZ-1164), 10 ng/mL recombinant human IL-23 (Proteintech HZ-1254), 10 ug/mL anti-IL4 (BioXCell BE0240, clone MP4-25D2), and 10 ug/mL anti-IFNγ (BioXCell BE0235, clone B133.5)) at a density of 1 million cells per mL. ..

    Article Title: A20’s Linear Ubiquitin Binding Motif Restrains Pathogenic Activation of TH17/22 cells and IL-22 Driven Enteritis
    Article Snippet: Naïve CD4 T cells were isolated from murine splenocytes using a Mouse CD4 naïve T cell negative selection kit (StemCell Technologies 19765) per manufacturer’s instructions. .. Isolated cells were subsequently differentiated in plates pre-coated with 2 ug/mL anti-CD3 (overnight at 4 C, or 2 h at 37 C) in TH17 medium (IMDM with 10% FBS, 50 uM beta-mercaptoethanol, 2 ug/mL anti-CD28 (BioXCell BE0015, clone 37.51), 20 ng/mL recombinant murine IL-6 (R&D Biosystems 406-ML), 5 ng/mL recombinant human TGFb1 (PeproTech 100-21), 10 ug/mL anti-IL4 (BioXCell BE0045, clone 11B11), and 10 ug/mL anti-IFNg (BioXCell BE0055, clone XMG1.2)) at a density of 1 million cells per mL. ..

    Cell Differentiation:

    Article Title: Sensitive Transcriptomics and Genotyping reveals function of genetic variants in immunity
    Article Snippet: For CD4+ or CD8+ effector/memory differentiation, the medium was also supplemented with 10 ng/ml hIL-2 (R&D Systems, BT-002-050), 5 ng/ml of hIL-7 (R&D Systems, BT-007-AFL-025), 5 ng/ml hIL-15 (R&D Systems, BT-015-AFL-025) . .. For CD4+ regulatory Th1 cell differentiation, the medium was supplemented with 10 ng/ml hIL-2, 5 ng/ml hIL-12 (R&D Systems, 10018-IL-010) and 1 ug/mL anti-IL4 (BioXcell, BE0240) . .. T cells were activated with anti-CD3/CD28 Dynabeads at 1:1 ratio of the cells (ThermoFisher, 11131D) for 2 days in the complete XVivo15 medium with appropriate cytokines.

    In Vivo:

    Article Title: Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma
    Article Snippet: .. To assess the role of IL4 in macrophage differentiation in vivo , CD4CreER T2 Vav1 WT/co–Vav1-Myo1f mice were treated with 1.5 mg of anti-IL4 (11B11, BioXCell) or anti-HRP control (BE0088, BioXCell) in 200 μL of PBS injected intraperitoneal. ..

    Control:

    Article Title: Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma
    Article Snippet: .. To assess the role of IL4 in macrophage differentiation in vivo , CD4CreER T2 Vav1 WT/co–Vav1-Myo1f mice were treated with 1.5 mg of anti-IL4 (11B11, BioXCell) or anti-HRP control (BE0088, BioXCell) in 200 μL of PBS injected intraperitoneal. ..

    Injection:

    Article Title: Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma
    Article Snippet: .. To assess the role of IL4 in macrophage differentiation in vivo , CD4CreER T2 Vav1 WT/co–Vav1-Myo1f mice were treated with 1.5 mg of anti-IL4 (11B11, BioXCell) or anti-HRP control (BE0088, BioXCell) in 200 μL of PBS injected intraperitoneal. ..

    Isolation:

    Article Title: A20’s Linear Ubiquitin Binding Motif Restrains Pathogenic Activation of TH17/22 cells and IL-22 Driven Enteritis
    Article Snippet: Naïve CD4 T cells were isolated from murine splenocytes using a Mouse CD4 naïve T cell negative selection kit (StemCell Technologies 19765) per manufacturer’s instructions. .. Isolated cells were subsequently differentiated in plates pre-coated with 2 ug/mL anti-CD3 (overnight at 4 C, or 2 h at 37 C) in TH17 medium (IMDM with 10% FBS, 50 uM beta-mercaptoethanol, 2 ug/mL anti-CD28 (BioXCell BE0015, clone 37.51), 20 ng/mL recombinant murine IL-6 (R&D Biosystems 406-ML), 5 ng/mL recombinant human TGFb1 (PeproTech 100-21), 10 ug/mL anti-IL4 (BioXCell BE0045, clone 11B11), and 10 ug/mL anti-IFNg (BioXCell BE0055, clone XMG1.2)) at a density of 1 million cells per mL. ..

    In Vitro:

    Article Title: Type I IFN signaling in the absence of IRGM1 promotes M. tuberculosis replication in immune cells by suppressing T cell responses.
    Article Snippet: Naik et al. Mucosal Immunology xxx (xxxx) xxx Activated T cell transfer and neutrophil depletion ESAT6-specific C7 T cells were isolated from lymph nodes and spleen from C7 TCR tg/thy1.1 transgenic mice with Miltenyi Biotec CD4+ T cell isolation kit (130–104-454) and the cell purity (95–97 %) was assessed by flow cytometry. .. C7 T cells were activated in vitro with ESAT-6 (5 μg/ ml), anti-IL4 (BioXcell #BE0045 clone 11B11;5μg/ml), and rIL12 (20 ng/ml) for two days followed by co-culturing with 50U/ml IL-2 for an additional two days. ..

    Article Title: Type I IFN signaling in the absence of IRGM1 promotes M. tuberculosis replication in immune cells by suppressing T cell responses
    Article Snippet: ESAT6-specific C7 T cells were isolated from lymph nodes and spleen from C7 TCR tg/thy1.1 transgenic mice with Miltenyi Biotec CD4 + T cell isolation kit (130-104-454) and the cell purity (95-97%) was assessed by flow cytometry. .. C7 T cells were activated in vitro with ESAT-6 (5μg/ml), anti-IL4 (BioXcell #BE0045 clone 11B11;5μg/ml), and rIL12 (20ng/ml) for two days followed by co-culturing with 50U/ml IL-2 for an additional two days. ..

    other:

    Article Title: Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanism
    Article Snippet: For Aid-EYFP mice, tamoxifen (Cayman chemical) was resuspended in corn oil (Sigma), sonicated and given by oral gavage at 5mg/100μl.



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